Chelsea left-back Chilwell, who missed Sunday’s win over Belgium, has left the camp due to a pre-existing foot injury.Atletico Madrid defender Trippier has been given leave due to personal reasons.
That leaves 28 players for consideration for Gareth Southgate’s 23-man matchday squad.
With both missing, it is likely Southgate will turn to one of two Arsenal players at his disposal – with Bukayo Saka and Ainsley Maitland-Niles both adept down the left.
Captain Harry Kane is expected to be fit to start having been a second-half substitute at the weekend as England go in search of the win that will leave qualification to the Nations League finals in their own hands.
Trippier captained England in their 3-0 friendly win over Wales last week and also started the 2-1 victory against Belgium.